The present manuscript describes simple, sensitive, rapid, accurate, precise and economical ratio spectra derivative method for the simultaneous determination of paracetamol and tramadol HCl in tablet dosage form. The method is based on use of the first derivative of the ratio of spectra. The absorption spectrum of the mixture is obtained and the amplitudes at appropriate wavelengths are divided by the corresponding amplitudes in the absorption spectrum of a standard solution of one of the components and the first derivative of the ratio spectrum is obtained. The concentration of the other component is then determined from a calibration graph. The amplitude in first derivative of ratio spectra at 236.20 nm and 266.40 nm were selected to determine paracetamol and tramadol HCl, respectively in distilled water. The linearity was obtained in the concentration range of 2-14 μg/ml for paracetamol and 10-22 μg/ml for tramadol HCl. The method was successfully applied for the determination of these two drugs in tablet. No interference was observed from excipients present in the tablet dosage form. The suitability of this method for the quantitative determination of paracetamol and tramadol HCl was proved by validation. The proposed method was found to be simple and sensitive for the routine analysis of these two drugs in tablet dosage form. The results of analysis have been validated statistically and by recovery studies.
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